Pepsin preparation.



UNITED STATES PATENT OFFICE.

JULIUS ALTS'oHUL, OF BERLIN, GERMANY, ASSIGNoR'To AOTIEN-GESELL- SOVHAFTMFUR ANILIN FABRIKATION, OF BERLIN, GERMANY.

PEPSIN PREPARATION.

rations of Pepsin Containing Hydrochloric Acid; and I do hereby declare that the followingisa full, clear, and exact description thereof, which will enable others skilled in the art to which it appertains to make and use the same.

Pepsin and hydrochloric acid are known to be the most important agents in gastric digestion; but all attempts to make satisfactory preparations that shall contain boththese agents have failed. Mixtures of the two can-v I not be preserved. Moreover, in order to make a dry preparation containing any considerable'proportion of aqueous hydrochloric acid an' inconveniently-large proportion of some third substance must be introduced into the mixture to act as an absorbent of the liquid.

This invention relates to a process for themanufacture of stable preparations of pepsin containing hydrochloric acid.

The invention depends upon the fact that the hydrochlorids of aliphatic amido derivatives, such as amido acids and their 'alkyl derivatives or their esters, are so completely hydrolyzed by water that when they are dissolved or mixed with water the latter acquires the properties of hydrochloric acid, among which is the power to digest proteid in conjunction with pepsin. To apply this fact for making preparations of pepsin containing hydrochloric acid, the pepsin is mixed with a hydrochlorid of an aliphatic amido derivative which has no ill effect on the animal organism, such as betain hydrochlorid, glycocin hydrochlorid, alanin hydrochlorid, or leucin hydrochlorid. In the presence of water a mixture of this kind has all the digestive properties of a mixtureof pepsin and hydrochloric acid. The hydrochlorids in question being solid substances may be ground with any proportion of pepsin, and the mixture is perfectly stable and can be made up into any desired Specification of Letters Patent. Application filed July 8,1905. Serial n. 268,870.

iPatented Dec. 5, 1905.

form, such as pills or tabloids, which can be preserved without losing the property of digesting proteids in presence of water.

In practicing the invention the pepsin and the hydrochloridfor instance, betain hydrochloridare ground together in such proportions that the mixture will contain the desired percentage of hydrochloric acid. For example, if this percentage is to be ten per cent.

four parts of betain hydrochlorid are ground with six parts of pepsin, or to make a preparation containing twenty per cent. of pepsin and twenty per cent. of hydrochloric acid eight parts of betain hydrochlorid are ground with two. parts of pepsin. If it merely be desired to obtain a pepsin which is soluble in water, a much smaller proportion of the by- For instance, when.

drochlorid will suffice. the latter is betain hydrochloridtwo and one-half parts of it may be mixed with ninetythe hydrochlorid of an aliphatic amido derivative which has no ill effect upon the animal organism, which preparation is stable when preserved and is capable of digesting proteids in the presence of water.

2. As a new articleof manufacture a preparation consisting of a mixture of betain hydrochlorid and pepsin, which preparation is stable when preserved and is capable of digesting proteids in the presence of water.

, In Witness whereof I have hereunto signed my name, this 24th day of June, 1905, in the presence of two subscribing witnesses.

JULIUS ALTSCHUL. Witnesses:

HENRY HASPER,

WOLDEMAR, 'HAUPT. 

